概述

  • 产品名称Anti-UHRF1抗体
    参阅全部 UHRF1 一抗
  • 描述
    小鼠单克隆抗体to UHRF1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: WNEVLASLKD RPASGSPFQL FLSKVEETFQ CICCQELVFR PITTVCQHNV CKDCLDRSFR AQVFSCPACR YDLGRSYAMQ VNQPLQTVLN QLFPGYGNGR , corresponding to amino acids 694-794 of Human UHRF1

性能

应用

Our Abpromise guarantee covers the use of ab57083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
  • 组织特异性Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • 发展阶段Expressed in fetal thymus, liver and kidney.
  • 结构域The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.
  • 翻译后修饰Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ac2-121 antibody
    • AL022808 antibody
    • E3 ubiquitin-protein ligase UHRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ21925 antibody
    • hNP95 antibody
    • hUHRF1 antibody
    • HuNp95 antibody
    • ICBP90 antibody
    • Inverted CCAAT box binding protein of 90 kDa antibody
    • Inverted CCAAT box binding protein, 90-kD antibody
    • Inverted CCAAT box-binding protein of 90 kDa antibody
    • Liver regeneration-related protein LRRG126 antibody
    • MGC138707 antibody
    • NP95 antibody
    • Nuclear phosphoprotein, 95-KD antibody
    • Nuclear protein 95 antibody
    • Nuclear zinc finger protein Np95 antibody
    • RING finger protein 106 antibody
    • RNF106 antibody
    • Transcription factor ICBP90 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
    • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
    • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
    • Ubiquitin-like with PHD and ring finger domains 1 antibody
    • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
    • UHRF1 antibody
    • UHRF1_HUMAN antibody
    see all

Anti-UHRF1 antibody 图像

  • UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.


  • Predicted band size : 90 kDa
    UHRF1 antibody (ab57083) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-UHRF1 antibody (ab57083)参考文献

This product has been referenced in:
  • Wang F  et al. UHRF1 Promotes Cell Growth and Metastasis Through Repression of p16(ink4a) in Colorectal Cancer. Ann Surg Oncol : (2012). Read more (PubMed: 22219067) »
  • Rothbart SB  et al. Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation. Nat Struct Mol Biol 19:1155-60 (2012). WB . Read more (PubMed: 23022729) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 boiling for 20 minutes in a MW oven at 600W
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Sep 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
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提交于 Jan 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"