概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to UHRF1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: WNEVLASLKD RPASGSPFQL FLSKVEETFQ CICCQELVFR PITTVCQHNV CKDCLDRSFR AQVFSCPACR YDLGRSYAMQ VNQPLQTVLN QLFPGYGNGR , corresponding to amino acids 694-794 of Human UHRF1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab213223 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab57083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
  • 组织特异性
    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • 发展阶段
    Expressed in fetal thymus, liver and kidney.
  • 结构域
    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.
  • 翻译后修饰
    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ac2-121 antibody
    • AL022808 antibody
    • E3 ubiquitin-protein ligase UHRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ21925 antibody
    • hNP95 antibody
    • hUHRF1 antibody
    • HuNp95 antibody
    • ICBP90 antibody
    • Inverted CCAAT box binding protein of 90 kDa antibody
    • Inverted CCAAT box binding protein, 90-kD antibody
    • Inverted CCAAT box-binding protein of 90 kDa antibody
    • Liver regeneration-related protein LRRG126 antibody
    • MGC138707 antibody
    • NP95 antibody
    • Nuclear phosphoprotein 95 antibody
    • Nuclear phosphoprotein, 95-KD antibody
    • Nuclear protein 95 antibody
    • Nuclear zinc finger protein Np95 antibody
    • RING finger protein 106 antibody
    • RNF106 antibody
    • TDRD22 antibody
    • Transcription factor ICBP90 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
    • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
    • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
    • Ubiquitin-like with PHD and ring finger domains 1 antibody
    • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
    • UHRF1 antibody
    • UHRF1_HUMAN antibody
    see all

图片

  • UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.
  • UHRF1 antibody (ab57083) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Yao J  et al. Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus. J Clin Invest 126:732-44 (2016). Read more (PubMed: 26752645) »
  • Abu-Alainin W  et al. UHRF1 regulation of the Keap1-Nrf2 pathway in pancreatic cancer contributes to oncogenesis. J Pathol 238:423-33 (2016). Read more (PubMed: 26497117) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 boiling for 20 minutes in a MW oven at 600W
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult testis)
Specification
Adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

煜欽 林

Verified customer

提交于 Jan 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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