概述

  • 产品名称
  • 描述
    兔多克隆抗体to UHRF1
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 275-325 of Human UHRF1 (NP_001041666.1)

  • 阳性对照
    • Human Bladder Cell Carcinoma, Human Breast Carcinoma, Human Skin Basal Cell Carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab100810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
  • 组织特异性
    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • 发展阶段
    Expressed in fetal thymus, liver and kidney.
  • 结构域
    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.
  • 翻译后修饰
    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ac2-121 antibody
    • AL022808 antibody
    • E3 ubiquitin-protein ligase UHRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ21925 antibody
    • hNP95 antibody
    • hUHRF1 antibody
    • HuNp95 antibody
    • ICBP90 antibody
    • Inverted CCAAT box binding protein of 90 kDa antibody
    • Inverted CCAAT box binding protein, 90-kD antibody
    • Inverted CCAAT box-binding protein of 90 kDa antibody
    • Liver regeneration-related protein LRRG126 antibody
    • MGC138707 antibody
    • NP95 antibody
    • Nuclear phosphoprotein 95 antibody
    • Nuclear phosphoprotein, 95-KD antibody
    • Nuclear protein 95 antibody
    • Nuclear zinc finger protein Np95 antibody
    • RING finger protein 106 antibody
    • RNF106 antibody
    • TDRD22 antibody
    • Transcription factor ICBP90 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
    • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
    • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
    • Ubiquitin-like with PHD and ring finger domains 1 antibody
    • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
    • UHRF1 antibody
    • UHRF1_HUMAN antibody
    see all

图片

  • Staining of Human UHRF1 in a Formalin/PFA-fixed paraffin-embedded section of Human Basal Cell Carcinoma using ab100810 at a dilution of 1/250.
  • Staining of Human UHRF1 in a Formalin/PFA-fixed paraffin-embedded section of Human Breast Carcinoma using ab100810 at a dilution of 1/250.

文献

This product has been referenced in:
  • Nascimento EM  et al. The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nat Cell Biol 13:1395-405 (2011). IHC . Read more (PubMed: 22101514) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.

In general, as negative control the following could be used:

-a tissue that does not express the protein (see HumanProtein Atlas- spleen tissue:
http://www.proteinatlas.org/ENSG00000034063/normal,...

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Thank you for contacting us with your enquiry.

Ab27478 is a suitable isotype control to use with an unconjugated rabbit polyclonal primary antibody, so I would recommend using it as a negative controlfor ab100810 and ab7046 in IHC. As the IgG...

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