Anti-UGT8抗体(ab84288)

概述

  • 产品名称Anti-UGT8抗体
    参阅全部 UGT8 一抗
  • 描述
    兔多克隆抗体to UGT8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (GILLEWKTVT EKELYEALVK VINNPSYRQR AQKLSEIHKD QPGHPVNRTI) of Human UGT8 (NP_003351).

  • 阳性对照
    • Human fetal heart lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
  • 通路Sphingolipid metabolism; galactosylceramide biosynthesis.
  • 序列相似性Belongs to the UDP-glycosyltransferase family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2 hydroxyacylsphingosine 1 beta galactosyltransferase antibody
    • 2-hydroxyacylsphingosine 1-beta-galactosyltransferase antibody
    • Ceramide UDP galactosyltransferase antibody
    • Ceramide UDP-galactosyltransferase antibody
    • Cerebroside synthase antibody
    • CGT antibody
    • CGT_HUMAN antibody
    • mCerGT antibody
    • UDP galactose ceramide galactosyltransferase antibody
    • UDP galactose ceramide galactosyltransferase 8 antibody
    • UDP glycosyltransferase 8 antibody
    • UDP-galactose-ceramide galactosyltransferase antibody
    • UGT4 antibody
    • Ugt8 antibody
    • Uridine diphosphate glycosyltransferase 8 antibody
    see all

Anti-UGT8 antibody 图像

  • Anti-UGT8 antibody (ab84288) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

Anti-UGT8 antibody (ab84288)参考文献

ab84288 has not yet been referenced specifically in any publications.

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Lin Zhang

Verified customer

提交于 Feb 13 2012

Thank you for contacting Abcam.

I am happy that you were able to get some good staining for ab84288. When you submit the Abreview, you should estimate the size the extra bands to the best of your ability, so if it is between 42-50, then you c...

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DISCOUNT CODE: ************
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab84288 in mouse samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To r...

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Thank you for your reply.

Both antibodies are similar, with no real difference between them. We carry multiply antibodies to the same targets in our catalogue.

As neither have been tested in mouse tissue, but both are predicted to w...

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Thank you for contacting Abcam.

I have to admit to that I am not an expert of the function of UGT8 and so I can only go with the information on the website, but it does appear that UGT8 catalyses the following reaction:

UDP-galactos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"