• 产品名称Anti-UGT2B17抗体
  • 描述
    兔多克隆抗体to UGT2B17
  • 特异性The immunogen has 92% homology with UGT2B15, and the overall homology between UGT2B15 and UGT2B17 is 94%. We have not tested cross reactivity of this antibody with UGT2B15 and welcome any feedback.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the center region of Human UGT2B17.

  • 阳性对照
    • Y79 cell line lysates




Our Abpromise guarantee covers the use of ab92610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 61 kDa.Can be blocked with UGT2B17 peptide (ab156833).
ELISA 1/1000.


  • 相关性UGT2B17 belongs to the family of UDP-glucuronosyltransferases (UGTs; EC, enzymes that catalyze the transfer of glucuronic acid from uridine diphosphoglucuronic acid to a variety of substrates, including steroid hormones.
  • 细胞定位Microsome membrane; Single pass membrane protein. Endoplasmic reticulum membrane; Single pass membrane protein.
  • 数据库链接
  • 别名
    • C19 steroid specific UDP glucuronosyltransferase antibody
    • C19 steroid specific UDPGT antibody
    • UDB17 antibody
    • UDP glucuronosyltransferase 2B17 antibody
    • UDPGT 2B17 antibody
    see all

Anti-UGT2B17 antibody 图像

  • Anti-UGT2B17 antibody (ab92610) at 1/50 dilution + Y79 cell line lysates at 35 µg

    Predicted band size : 61 kDa
  • ICC/IF image of ab92610 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92610, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab92610 staining UGT2B17 in LNCaP Androgen Dependent Prostate Cancer cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in Triton X-100 prior to blocking in 5% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 in PBS/1%BSA and incubated with the sample for 24 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal, diluted 1/250. Blue nuclear counterstain is Hoechst staining.

    See Abreview

Anti-UGT2B17 antibody (ab92610)参考文献

ab92610 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP Androgen Dependent Prostate Cancer Cells)
Specification LNCaP Androgen Dependent Prostate Cancer Cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Skyla Carney

Verified customer

提交于 Jul 29 2011

Thank you for your enquiry. I can confirm that according to the Uniprot protein database page (link on the datasheet), the UGT2B17 protein has a molecular wieght of 61 kDa, and this protein should be detected around this size. UniProt page URL ad...

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