概述

  • 产品名称Anti-UGT1A3抗体
  • 描述
    小鼠单克隆抗体to UGT1A3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: KVLVVPIDGS HWLSMREVLR ELHARGHQAV VLTPEVNMHI KEENFFTLTT YAISWTQDEF DRHVLGHTQL YFETEHFLKK FFRSMAMLNN MSLVYHRSCV , corresponding to amino acids 30-130 of Human UGT1A3

性能

应用

Our Abpromise guarantee covers the use of ab57400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The UGT enzymes comprise a superfamily of integral membrane proteins of the endoplasmic reticulum that have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
    • 细胞定位Microsome. Endoplasmic reticulum; Single-pass membrane protein.
    • 数据库链接
    • 别名
      • EC 2.4.1.17 antibody
      • GNT 1 antibody
      • GNT1 antibody
      • UDP glucuronosyltransferase 1 3 antibody
      • UDP glucuronosyltransferase 1 family polypeptide A3 antibody
      • UDP glucuronosyltransferase 1A3 antibody
      • UDP glucuronosyltransferase antibody
      • UDP glycosyltransferase 1 family polypeptide A3 antibody
      • UDPGT antibody
      • UGT 1 antibody
      • UGT 1C antibody
      • UGT1 antibody
      • UGT1 03 antibody
      • UGT1*3 antibody
      • UGT1.3 antibody
      • UGT1C antibody
      see all

    Anti-UGT1A3 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57400 UGT1A3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-UGT1A3 antibody (ab57400)参考文献

    This product has been referenced in:
    • Collier AC  et al. UDP-glucuronosyltransferase 1a enzymes are present and active in the mouse blastocyst. Drug Metab Dispos 42:1921-5 (2014). Read more (PubMed: 25200869) »
    • Jarrar YB  et al. Determination of major UDP-glucuronosyltransferase enzymes and their genotypes responsible for 20-HETE glucuronidation. J Lipid Res 55:2334-42 (2014). Read more (PubMed: 25249502) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Ovary)
    Antigen retrieval step Enzymatic
    Permeabilization No
    Specification Ovary
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 02 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (Membranes from insect cells)
    Loading amount 60000 µg
    Specification Membranes from insect cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Moshe Finel

    Verified customer

    提交于 Oct 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"