The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 177 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt,ICC or IP.
Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
Higher levels in pancreas, skeletal muscle, kidney, and brain. Low levels in lung and heart.
Protein modification; protein glycosylation.
Belongs to the glycosyltransferase 8 family.
The N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects.