The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 175 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
组织特异性Higher levels in pancreas, skeletal muscle, kidney, and brain. Low levels in lung and heart.
通路Protein modification; protein glycosylation.
序列相似性Belongs to the glycosyltransferase 8 family.
结构域The N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects.