概述

  • 产品名称Anti-UFM1抗体[EPR4264(2)]
    参阅全部 UFM1 一抗
  • 描述
    兔单克隆抗体[EPR4264(2)] to UFM1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues from C terminal of Human UFM1.

  • 阳性对照
    • Fetal kidney, 293T, SH-SY5Y, and HepG2 cell lysates; Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR4264(2)
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 9 kDa.
IP 1/10 - 1/40.
IHC-P 1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    Anti-UFM1 antibody [EPR4264(2)] 图像

    • ab109305 immunoprecipitating UFM1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/1000.

      Lane 1: 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
      Lane 2: 293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109305 in 293 (Human embryonic kidney epithelial cell) whole cell lysate

    • ab109305 staining UFM1 in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in mouse mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/2000 dilution

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 3 : Mouse spleen cell lysate
      Lane 4 : Mouse heart cell lysate
      Lane 5 : Rat spleen cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size : 9 kDa
      Observed band size : 9,28 kDa (why is the actual band size different from the predicted?)

      Mouse heart cell lysate Blocking and diluting buffer: 5% NFDM/TBST

      The band at around 28kDa might be UFM1 conjugates [PMID: 23152784].

    • All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
      Observed band size : 9 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.

    Anti-UFM1 antibody [EPR4264(2)] (ab109305)参考文献

    This product has been referenced in:
    • Gavin JM  et al. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. J Biol Chem 289:22648-58 (2014). Read more (PubMed: 24966333) »
    • Lee YJ  et al. Global Protein Conjugation by Ubiquitin-Like-Modifiers during Ischemic Stress Is Regulated by MicroRNAs and Confers Robust Tolerance to Ischemia. PLoS One 7:e47787 (2012). WB ; Other . Read more (PubMed: 23094087) »

    See all 2 Publications for this product

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