存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度 Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明 Is unsuitable for Flow Cyt or ICC.
功能 Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.
序列相似性 Belongs to the UFM1 family.
细胞定位 Nucleus. Cytoplasm. Predominantly nuclear. Also expressed diffusely in the cytoplasm.
Information by UniProt
BM 002 antibody
Anti-UFM1 antibody [EPR4264(2)] 图像
Western blot - UFM1 antibody [EPR4264(2)] (ab109305)
All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution Lane 1 : Fetal kidney lysate Lane 2 : 293T cell lysate Lane 3 : SH-SY5Y cell lysate Lane 4 : HepG2 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size : 9 kDa Observed band size : 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UFM1 antibody [EPR4264(2)] (ab109305)
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.
Anti-UFM1 antibody [EPR4264(2)] (ab109305)参考文献
This product has been referenced in:
et al. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. J Biol Chem 289:22648-58 (2014).
Read more (PubMed: 24966333) »
et al. Global Protein Conjugation by Ubiquitin-Like-Modifiers during Ischemic Stress Is Regulated by MicroRNAs and Confers Robust Tolerance to Ischemia. PLoS One 7:e47787 (2012).
Read more (PubMed: 23094087) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"