概述

  • 产品名称
  • 描述
    兔多克隆抗体to UFD1L
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell IHC-P: H441 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab96648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
  • 组织特异性
    Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
  • 通路
    Protein degradation; proteasomal ubiquitin-dependent pathway.
  • 序列相似性
    Belongs to the UFD1 family.
  • 细胞定位
    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • UB fusion protein 1 antibody
    • Ubiquitin fusion degradation 1 like (yeast) antibody
    • Ubiquitin fusion degradation 1 like antibody
    • Ubiquitin fusion degradation protein 1 homolog antibody
    • UFD1 antibody
    • UFD1_HUMAN antibody
    • UFD1L antibody
    see all

图片

  • All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 35 kDa
  • ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
    Lower image: merged with DNA probe
  • ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.

文献

ab96648 has not yet been referenced specifically in any publications.

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