Mouse, Rat, Cow, Zebrafish
Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).
WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates
ICC/IF: A431 cell
IHC-P: H441 xenograft
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
1/100 - 1/500.
1/100 - 1/200.
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Protein degradation; proteasomal ubiquitin-dependent pathway.
Belongs to the UFD1 family.
Nucleus. Cytoplasm > cytosol.
Information by UniProt
UB fusion protein 1 antibody
Ubiquitin fusion degradation 1 like (yeast) antibody
Ubiquitin fusion degradation 1 like antibody
Western blot - UFD1L antibody (ab96648)
All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 35 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - UFD1L antibody (ab96648)
ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UFD1L antibody (ab96648)
ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.
has not yet been referenced specifically in any publications.
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