The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Possible negative regulator of polyubiquitination.
组织特异性Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
序列相似性In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. In the C-terminal section; belongs to the LDH/MDH superfamily. Contains 1 UEV (ubiquitin E2 variant) domain.
ab92677 at 1/50 dilution staining UEVLD in (1) Human breast carcinoma in absence of blocking peptide and (2) Human breast carcinoma in presence of blocking peptide by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Anti-UEVLD antibody (ab92677)参考文献
has not yet been referenced specifically in any publications.