Anti-UCP3抗体[mAbcam61299] (ab61299)

概述

  • 产品名称
    Anti-UCP3抗体[mAbcam61299]
    参阅全部 UCP3 一抗
  • 描述
    小鼠单克隆抗体[mAbcam61299] to UCP3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Macaque monkey
  • 免疫原

    Synthetic peptide within Human UCP3 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P55916
    (Peptide available as ab100825)

  • 阳性对照
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab61299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • 组织特异性
    Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • 疾病相关
    Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式
    UCP3 is preferentially expressed in muscle
  • 别名
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

图片

  • Anti-UCP3 antibody [mAbcam61299] (ab61299) at 10 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgM - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

实验方案

文献

ab61299 has not yet been referenced specifically in any publications.

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