概述

  • 产品名称Anti-UCP3抗体
    参阅全部 UCP3 一抗
  • 描述
    兔多克隆抗体to UCP3
  • 特异性No cross-reactivity with UCP1 and UCP2.
  • 经测试应用适用于: WB, IHC-FoFr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human UCP3 aa 254-267 conjugated to keyhole limpet haemocyanin.
    Database link: P55916

  • 常规说明Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab10985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 34 kDa. Predicted molecular weight migrates at ~50 kDa in positive control lysate. (Protein is glycosylated)
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/3000.

靶标

  • 功能UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • 组织特异性Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • 疾病相关Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式UCP3 is preferentially expressed in muscle
  • 别名
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Anti-UCP3 antibody 图像

  • ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-UCP3 antibody (ab10985)参考文献

This product has been referenced in:
  • Becerril S  et al. Transcriptional analysis of brown adipose tissue in leptin-deficient mice lacking inducible nitric oxide synthase: evidence of the role of Med1 in energy balance. Physiol Genomics 44:678-88 (2012). WB ; Mouse . Read more (PubMed: 22570438) »
  • Becerril S  et al. Deletion of inducible nitric-oxide synthase in leptin-deficient mice improves brown adipose tissue function. PLoS One 5:e10962 (2010). WB, IHC-P ; Mouse . Read more (PubMed: 20532036) »

See all 3 Publications for this product

Product Wall

Thank you for sending on these details. The UCP3 protein that this antibody recognises undergoes a lot of post transcriptional modification events. The bands seen in the western blot are probably from the heavier glycosylated forms of the protein, whic...

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Thank you for contacting us and taking the time to fill out the questionaire. I am sorry you have had trouble using this rabbit polyclonal anti-UCP3 antibody (ab10985) I have had a look at your procedure and have come up with the following suggesti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Skeletal muscle (mouse))
Specification Skeletal muscle (mouse)
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
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提交于 Jan 18 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"