概述

  • 产品名称Anti-UCP1抗体
    参阅全部 UCP1 一抗
  • 描述
    兔多克隆抗体to UCP1
  • 经测试应用适用于: WB, IHC-P, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog
    预测可用于: Hamster, Human, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP1.

    (Peptide available as ab24282.)

  • 阳性对照
    • WB: Mouse and rat brown adipose tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab23841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/1000.

靶标

  • 功能UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • 组织特异性Brown adipose tissue.
  • 序列相似性Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式UCP1 is preferentially expressed in brown adipose tissue
  • 别名
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Anti-UCP1 antibody 图像

  • All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml

    Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
    Lane 2 : Adult Rat Brown Adipose Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.

     

Anti-UCP1 antibody (ab23841)参考文献

This product has been referenced in:
  • Stechschulte LA  et al. FKBP51 Null Mice Are Resistant to Diet-Induced Obesity and the PPAR? Agonist Rosiglitazone. Endocrinology N/A:en20151996 (2016). Read more (PubMed: 27442117) »
  • Tsai SY  et al. Increased 4E-BP1 Expression Protects against Diet-Induced Obesity and Insulin Resistance in Male Mice. Cell Rep 16:1903-14 (2016). Read more (PubMed: 27498874) »

See all 20 Publications for this product

Product Wall

Thank you for your inquiry.


We do not currently offer this antibody directly conjugated to a fluorophore.I could see if the lab would be able to generate this for you, but we do not carry Alexa Fluors. We may be able to provide this ser...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human pre-adipocytes overexpressing hUCP1)
Gel Running Conditions Non-reduced Denaturing (4-15%)
Loading amount 30 µg
Specification Human pre-adipocytes overexpressing hUCP1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. Morten Lundh

Verified customer

提交于 Jul 18 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cance cells)
Specification A549 Lung cance cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Feb 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10.5%)
Sample Mouse Tissue lysate - other (brown fat)
Specification brown fat
Treatment cold stress
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. KUN DONG

Verified customer

提交于 Jul 09 2013

Thank you for contacting us.

I've attached one of our blocking experiments with the antibody and peptide combination.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or i...

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I'm sending a free of charge vial of ab14571 on the order *** which should arrive on Tuesday, to replace the vial of ab47431. Please keep...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (brown adipose tissue)
Loading amount 40 µg
Specification brown adipose tissue
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Artery)
Specification Artery
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Feb 23 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"