概述

  • 产品名称Anti-UCKL1抗体
    参阅全部 UCKL1 一抗
  • 描述
    兔多克隆抗体to UCKL1
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Chimpanzee, Gorilla, Opossum, Chinese Hamster, Orangutan, Bat
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 50-100 of Human UCKL1 (NP_060329.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95177 was affinity purified using an epitope specific to UCKL1 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能May contribute to UTP accumulation needed for blast transformation and proliferation.
    • 组织特异性Ubiquitous.
    • 通路Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
      Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
    • 序列相似性Belongs to the uridine kinase family.
    • 翻译后修饰Ubiquitinated by RNF19B; which induces proteasomal degradation.
    • 细胞定位Cytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location.
    • Information by UniProt
    • 数据库链接
    • 别名
      • F538 antibody
      • UCK 1L antibody
      • UCK1 like antibody
      • UCK1L antibody
      • UCKL 1 antibody
      • UCKL1 antibody
      • UCKL1_HUMAN antibody
      • Uridine cytidine kinase 1 like 1 antibody
      • Uridine kinase like 1 antibody
      • Uridine-cytidine kinase-like 1 antibody
      • Uridine/cytidine kinase like 1 antibody
      • URKL 1 antibody
      • URKL1 antibody
      see all

    Anti-UCKL1 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UCKL1 with ab95177 at 1/1000 (1µg/mg). Detection: DAB.
    • Immunoprecipitation of UCKL1 from HeLa whole cell lysate (1 mg) using ab95177 at 3µg/mg lysate followed by Western blotting of 20% of the immunoprecipitate: (1) ab95177 at 1µg/ml, (2) control IgG
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-UCKL1 antibody (ab95177)参考文献

    ab95177 has not yet been referenced specifically in any publications.

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