概述

  • 产品名称Anti-UCHL3抗体
    参阅全部 UCHL3 一抗
  • 描述
    兔多克隆抗体to UCHL3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (EGQRWLPLEA NPEVTNQFLK QLGLHPNWQF VDVYGMDPEL LSMVPRPVCA) of Human UCHL3 (NP_005993).

  • 阳性对照
    • MCF7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
  • 组织特异性Highly expressed in heart, skeletal muscle, and testis.
  • 序列相似性Belongs to the peptidase C12 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
    • Ubiquitin thioesterase L3 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L3 antibody
    • UCH L3 antibody
    • UCH-L3 antibody
    • UCHL3 antibody
    • UCHL3_HUMAN antibody
    see all

Anti-UCHL3 antibody 图像

  • Anti-UCHL3 antibody (ab87154) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab87154 at 5 µg/ml staining UCHL3 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-UCHL3 antibody (ab87154)参考文献

ab87154 has not yet been referenced specifically in any publications.

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