兔单克隆抗体[EPR4896] to UCH37
Rat, Human 不与反应:
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
1/10 - 1/100.
1/500 - 1/1000. Antigen retrieval is recommended.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC.
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
Belongs to the peptidase C12 family.
Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
Information by UniProt
CGI 70 antibody
Western blot - Anti-UCH37 antibody [EPR4896] (ab124931)
All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution Lane 1 : Human fetal kidney cell lysates Lane 2 : 293T cell lysates Lane 3 : NCCIT cell lysates Lane 4 : SH-SY5Y cell lysates Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCH37 antibody [EPR4896] (ab124931)
ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.
This product has been referenced in:
et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018).
Read more (PubMed: 29353883) »
et al. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem 291:8773-83 (2016).
Read more (PubMed: 26907685) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"