重组Anti-UCH37抗体[EPR4896] (ab124931)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4896] to UCH37
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UCH37抗体[EPR4896]
参阅全部 UCH37 一抗 -
描述
兔单克隆抗体[EPR4896] to UCH37 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Rat不与反应: Mouse -
免疫原
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
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阳性对照
- Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4896 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124931于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
靶标
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功能
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. -
序列相似性
Belongs to the peptidase C12 family. -
细胞定位
Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 51377 Human
- Entrez Gene: 360853 Rat
- Omim: 610667 Human
- SwissProt: Q9Y5K5 Human
- Unigene: 591458 Human
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别名
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
see all
图片
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All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysates
Lane 2 : 293T cell lysates
Lane 3 : NCCIT cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab124931 被引用在 10 文献中.
- Hameed DS et al. Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor. Front Mol Biosci 9:866467 (2022). PubMed: 35720124
- Davies CW et al. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W. Nat Commun 12:4608 (2021). PubMed: 34326324
- Xiao M et al. MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma. Clin Transl Med 10:e241 (2020). PubMed: 33377647
- Liao Y et al. USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discov 5:24 (2019). PubMed: 31044085
- Sha B et al. Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma. Apoptosis 24:826-836 (2019). PubMed: 31342239
- Elu N et al. Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination. Front Physiol 10:534 (2019). PubMed: 31130875
- Jiang L et al. Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma. J Exp Clin Cancer Res 38:453 (2019). PubMed: 31694672
- Liao Y et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018). Human . PubMed: 29353883
- Lee JH et al. Inactivation of USP14 Perturbs Ubiquitin Homeostasis and Delays the Cell Cycle in Mouse Embryonic Fibroblasts and in Fruit Fly Drosophila. Cell Physiol Biochem 47:67-82 (2018). PubMed: 29763934
- Randles L et al. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem 291:8773-83 (2016). PubMed: 26907685