Anti-UBXN6抗体[5C3-1] (ab80659)

概述

  • 产品名称
    Anti-UBXN6抗体[5C3-1]
    参阅全部 UBXN6 一抗
  • 描述
    小鼠单克隆抗体[5C3-1] to UBXN6
  • 特异性
    ab80659 recognizes both isoforms of UBXN6
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant Human UBXN6 purified from bacteria.

  • 阳性对照
    • U2OS cotransfected with pBabepuro and pSuper based plasmid encoding anti UBXD1 shRNA

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 0.5% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    5C3-1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 0.05 - 0.5 µg/ml.
    IP: Use at 1-2µg/500 3µg protein extract.
    WB: Use at a concentration of 0.3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.
    • 组织特异性
      Enhanced expression in testis.
    • 序列相似性
      Contains 1 PUB (PUG) domain.
      Contains 1 UBX domain.
    • 结构域
      The UBX domain lacks key residues critical for VCP binding.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    Anti-UBXN6 antibody [5C3-1] 图像

    • Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)
      Lanes 3 - 4 : Ponceau S Red

      Lane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 2 : U2OS were co-transfected with mismatch control
      Lane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 4 : U2OS were co-transfected with mismatch control


      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 49 kDa (possible isoform).A picture of the Ponceau S stained blot after processing is shown to demonstrate equivalent loading of protein.

    Anti-UBXN6 antibody [5C3-1] (ab80659)参考文献

    ab80659 has not yet been referenced specifically in any publications.

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