概述

  • 产品名称
  • 描述
    兔多克隆抗体to UBR4
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 5133-5183 of Human UBR4 (NP_065816.1)

  • 阳性对照
    • HeLa whole cell lysate
  • 常规说明
    Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

应用

Our Abpromise guarantee covers the use of ab86738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 574 kDa.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
    • 通路
      Protein modification; protein ubiquitination.
    • 序列相似性
      Belongs to the UBR4 family.
      Contains 1 UBR-type zinc finger.
    • 细胞定位
      Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 600 kDa retinoblastoma protein associated factor antibody
      • 600 kDa retinoblastoma protein-associated factor antibody
      • E3 ubiquitin-protein ligase UBR4 antibody
      • KIAA0462 antibody
      • KIAA1307 antibody
      • N-recognin-4 antibody
      • p600 antibody
      • RBAF600 antibody
      • Retinoblastoma associated factor 600 like protein antibody
      • Retinoblastoma associated factor of 600 kDa antibody
      • Retinoblastoma-associated factor 600 antibody
      • Retinoblastoma-associated factor of 600 kDa antibody
      • Ubiquitin protein ligase E3 component n recognin 4 antibody
      • UBR4 antibody
      • UBR4_HUMAN antibody
      • Zinc finger UBR1 type protein 1 antibody
      • Zinc finger UBR1-type protein 1 antibody
      • Zinc finger, UBR1 type 1 antibody
      • ZUBR 1 antibody
      • ZUBR1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4 with ab86738 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-UBR4 antibody (ab86738) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 574 kDa


      Exposure time : 30 seconds

    文献

    This product has been referenced in:
    • Besche HC  et al. Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates. EMBO J 33:1159-76 (2014). Read more (PubMed: 24811749) »
    • Morrison J  et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . Read more (PubMed: 23555265) »

    See all 4 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
    Username

    Dr. James Dachtler

    Verified customer

    提交于 Jul 22 2015

    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Whole abdomen)
    Specification
    Whole abdomen
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Paromita Gupta

    Verified customer

    提交于 Nov 25 2014

    Thank you for contacting us regarding ab86738. I am sorry that you have been experiencing difficulties with this antibody in WB. Attached please find a complete protocol as well as the gel type information. I hope this information is helpful. P...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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