Anti-UBR4/p600抗体(ab114109)
Key features and details
- Rabbit polyclonal to UBR4/p600
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UBR4/p600抗体
参阅全部 UBR4/p600 一抗 -
描述
兔多克隆抗体to UBR4/p600 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Chicken, Dog, Turkey, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan -
免疫原
Synthetic peptide corresponding to Human UBR4/p600 aa 150-200.
Database link: NP_065816.1 -
阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab114109 was affinity purified using an epitope specific to UBR4/p600 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use at 10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the UBR4 family.
Contains 1 UBR-type zinc finger. -
细胞定位
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility. - Information by UniProt
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数据库链接
- Entrez Gene: 23352 Human
- Entrez Gene: 69116 Mouse
- Entrez Gene: 313658 Rat
- Omim: 609890 Human
- SwissProt: Q5T4S7 Human
- SwissProt: A2AN08 Mouse
- SwissProt: Q2TL32 Rat
- Unigene: 148078 Human
see all -
别名
- 600 kDa retinoblastoma protein associated factor antibody
- 600 kDa retinoblastoma protein-associated factor antibody
- E3 ubiquitin-protein ligase UBR4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4/p600 with ab114109 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human UBR4/p600 by Western blot of Immunoprecipitate.
UBR4/p600 was immunoprecipitated from HeLa whole cell lysate, using ab114109 at 10 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-UBR4/p600 antibody was used for the subsequent WB at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab114109 被引用在 1 文献中.
- Rinschen MM et al. The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes. Hum Mol Genet 25:1328-44 (2016). PubMed: 26792178