Detects endogenous levels of total UBQLN4 protein.
Synthetic peptide derived from internal sequence of Human UBQLN4 (UniProt ID: Q9NRR5).
Mouse brain lysate and Human colon carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
WB: 1/500 - 1/1000. Predicted molecular weight: 64 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
Contains 1 UBA domain.
Contains 1 ubiquitin-like domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm. Endoplasmic reticulum. May associate with the endoplasmic reticulum.
Information by UniProt
28S ribosomal protein S7 antibody
Western blot - UBQLN4 antibody (ab92674)
All lanes : Anti-UBQLN4 antibody (ab92674) at 1/500 dilution Lane 1 : Mouse brain lysate without blocking peptide Lane 2 : Mouse brain lysate with blocking peptide at 10µg Lysates/proteins at 30 µg per lane. Predicted band size : 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBQLN4 antibody (ab92674)
ab92674 at 1/50 dilution staining UBQLN4 in (1) Human colon carcinoma in absence of blocking peptide and (2) Human colon carcinoma in presence of blocking peptide by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
has not yet been referenced specifically in any publications.
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