概述

  • 产品名称Anti-Ubiquitin抗体[P4G7-H11]
    参阅全部 Ubiquitin 一抗
  • 描述
    小鼠单克隆抗体[P4G7-H11] to Ubiquitin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Cow, Human, Schizosaccharomyces pombe
  • 免疫原

    Native bovine Ubiquitin

  • 阳性对照
    • HeLa, RK-13, 3T3, PC12 and MDBK cell lysates; Mouse and Rat brain tissue extracts

性能

应用

Our Abpromise guarantee covers the use of ab90376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000. Detects a band of approximately 6.5 kDa (predicted molecular weight: 9 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • 细胞定位Cell Membrane, Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody [P4G7-H11] 图像

    • All lanes : Anti-Ubiquitin antibody [P4G7-H11] (ab90376) at 1/1000 dilution

      Lane 1 : Molecular weight marker
      Lane 2 : Ubiquitin
      Lane 3 : HeLa cell lysate
      Lane 4 : RK-13 cell lysate
      Lane 5 : 3T3 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : Mouse brain tissue extract
      Lane 8 : Rat brain tissue extract
      Lane 9 : MDBK cell lysate


      Predicted band size : 9 kDa

    Anti-Ubiquitin antibody [P4G7-H11] (ab90376)参考文献

    This product has been referenced in:
    • Szilagyi Z  et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol 32:2099-109 (2012). WB ; Schizosaccharomyces pombe . Read more (PubMed: 22451489) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"