Anti-Ubiquitin抗体(ab6181)

概述

  • 产品名称Anti-Ubiquitin抗体
    参阅全部 Ubiquitin 一抗
  • 描述
    羊多克隆抗体to Ubiquitin
  • 特异性Recognises both free ubiquitin and ubiquitin protein conjugates on western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.
  • 经测试应用适用于: Dot blot, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Human
  • 免疫原

    Ubiquitin from Sigma (Product number U6253) that was coupled to chicken gammaglobulin.

性能

应用

Our Abpromise guarantee covers the use of ab6181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
IHC-Fr
WB
  • 应用说明Immunohistochemistry: 1/2,000 to 5,000
    Western Blot: 1/1,000 to 2,000
    Immunoblot: 1/1,000 to 2,000
    1 site ELISA: Not tested.
    Biological Inhibition: Not tested.
  • 靶标

    • 相关性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • 细胞定位Cell Membrane, Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • FLJ25987 antibody
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    Anti-Ubiquitin antibody (ab6181)参考文献

    ab6181 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"