Anti-Ubiquitin +1抗体[40B3] (ab24259)

概述

  • 产品名称Anti-Ubiquitin +1抗体[40B3]
  • 描述
    小鼠单克隆抗体[40B3] to Ubiquitin +1
  • 经测试应用适用于: ELISA, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH

  • 阳性对照
    • Bosc23 cell lysate transfected with GFP Ub +1.

性能

应用

Our Abpromise guarantee covers the use of ab24259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IP
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml cell lysate.
    WB: 1/500 - 1/1000. Predicted molecular weight: 9 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Ubiquitin +1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially Ub+1 is accumulated in aggregates containing amyloid beta and phosphorylated tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also Ub+1 acts as an aggravating factor in polyglutamine induced neurodegeneration.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • UBB antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody

    Anti-Ubiquitin +1 antibody [40B3] (ab24259)参考文献

    ab24259 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - other (Human Patient Blood (EDTA preserved))
    Gel Running Conditions Reduced Denaturing (4-20% Bis-TRIS)
    Loading amount 1 µg
    Treatment TCA Precipitated
    Specification Human Patient Blood (EDTA preserved)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Mark Nakasone

    Verified customer

    提交于 Dec 01 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"