Anti-Ubiquinol-Cytochrome C Reductase Core蛋白I抗体[16D10AD9AH5] (ab110252)


  • 产品名称
    Anti-Ubiquinol-Cytochrome C Reductase Core蛋白I抗体[16D10AD9AH5]
    参阅全部 Ubiquinol-Cytochrome C Reductase Core Protein I 一抗
  • 描述
    小鼠单克隆抗体[16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core蛋白I
  • 宿主
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Bovine Ubiquinol-Cytochrome C Reductase Core Protein I protein.

  • 阳性对照
    • Human, Cow, Rat or Mouse heart mitochondrial lysate, HepG2 mitochondrial lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab110252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC/IF 1/50.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 53 kDa.



  • ab110252 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252) at 0.5 µg/ml

    Lane 1 : Human heart mitochondrial lysate
    Lane 2 : Cow heart mitochondrial lysate
    Lane 3 : Rat heart mitochondrial lysate
    Lane 4 : Mouse heart mitochondrial lysate
    Lane 5 : HepG2 mitochondrial lysate

    Predicted band size: 53 kDa

    Extra bands in the Mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Overlay histogram showing HepG2 cells stained with ab110252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Lim SC  et al. Loss of the Mitochondrial Fatty Acid ß-Oxidation Protein Medium-Chain Acyl-Coenzyme A Dehydrogenase Disrupts Oxidative Phosphorylation Protein Complex Stability and Function. Sci Rep 8:153 (2018). WB ; Human . Read more (PubMed: 29317722) »
  • Kazak L  et al. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Proc Natl Acad Sci U S A 114:7981-7986 (2017). WB ; Mouse . Read more (PubMed: 28630339) »

See all 47 Publications for this product


Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount
20 µg
Isolated Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Dec 05 2011