RabMAb

Anti-UBF1抗体[EPR2742] (ab109011)

概述

  • 产品名称Anti-UBF1抗体[EPR2742]
    参阅全部 UBF1 一抗
  • 描述
    兔单克隆抗体[EPR2742] to UBF1
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human UBF1.

  • 阳性对照
    • HeLa, A431, MCF7 and JAR cell lysates; Human colon and breast tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
    • 序列相似性Contains 6 HMG box DNA-binding domains.
    • 翻译后修饰Phosphorylated and activated by PIK3CA.
    • 细胞定位Nucleus > nucleolus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    Anti-UBF1 antibody [EPR2742] 图像

    • All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 89 kDa
    • ab109011 at 1/50 dilution staining UBF1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109011 at 1/50 dilution staining UBF1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue

    Anti-UBF1 antibody [EPR2742] (ab109011)参考文献

    ab109011 has not yet been referenced specifically in any publications.

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