The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
组织特异性Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
序列相似性Belongs to the ubiquitin conjugation factor E4 family. Contains 1 U-box domain.
翻译后修饰Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Detection of UBE4B by Western Blot of Immunprecipitate. ab72497 at 0.1µg/ml staining UBE4B in HeLa whole cell lysate immunoprecipitated using ab72501 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.
Anti-UBE4B antibody (ab72501)参考文献
has not yet been referenced specifically in any publications.