概述

  • 产品名称Anti-UBE3C抗体
    参阅全部 UBE3C 一抗
  • 描述
    小鼠多克隆抗体to UBE3C
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length UBE3C protein (Human)

  • 阳性对照
    • UBE3C transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab68225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 124 kDa).

靶标

  • 功能E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • 组织特异性Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • 结构域The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

Anti-UBE3C antibody 图像

  • All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilution

    Lane 1 : UBE3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 124 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

Anti-UBE3C antibody (ab68225)参考文献

ab68225 has not yet been referenced specifically in any publications.

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