概述

  • 产品名称Anti-UBE3C抗体
    参阅全部 UBE3C 一抗
  • 描述
    兔多克隆抗体to UBE3C
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 164-357 of Human UBE3C isoform 2 (AAH14029).

  • 阳性对照
    • HepG2 whole cell lysate; HeLa and HCT116 cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

性能

应用

Our Abpromise guarantee covers the use of ab101512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 124 kDa.

靶标

  • 功能E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • 组织特异性Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • 结构域The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

Anti-UBE3C antibody 图像

  • IHC image of UBE3C staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101512, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-UBE3C antibody (ab101512) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 124 kDa
    7.5% SDS-PAGE

Anti-UBE3C antibody (ab101512)参考文献

ab101512 has not yet been referenced specifically in any publications.

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