Anti-UBE3C抗体(ab101512)
Key features and details
- Rabbit polyclonal to UBE3C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UBE3C抗体
参阅全部 UBE3C 一抗 -
描述
兔多克隆抗体to UBE3C -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Recombinant fragment, corresponding to a region within amino acids 164-357 of Human UBE3C isoform 2 (AAH14029).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101512于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 124 kDa.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 124 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. -
组织特异性
Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 IQ domain. -
结构域
The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9690 Human
- Entrez Gene: 100763 Mouse
- Omim: 614454 Human
- SwissProt: Q15386 Human
- SwissProt: Q80U95 Mouse
- Unigene: 118351 Human
- Unigene: 137746 Mouse
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别名
- HectH2 antibody
- KIAA0010 antibody
- KIAA10 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab101512 被引用在 3 文献中.
- Hang C et al. Oncogenic UBE3C promotes breast cancer progression by activating Wnt/ß-catenin signaling. Cancer Cell Int 21:25 (2021). PubMed: 33407510
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Byrne R et al. Activity-Based Probes for HECT E3 Ubiquitin Ligases. Chembiochem 18:1415-1427 (2017). PubMed: 28425671