概述

  • 产品名称Anti-UBE3B抗体
    参阅全部 UBE3B 一抗
  • 描述
    兔多克隆抗体to UBE3B
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 720 - 769 (VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE) of Human UBE3B (NP_569733)

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
  • 数据库链接
  • 别名
    • AI449831 antibody
    • AU020130 antibody
    • FLJ45294 antibody
    • MGC131858 antibody
    • MGC78388 antibody
    • Ubiquitin protein ligase E3B antibody
    see all

Anti-UBE3B antibody 图像

  • Anti-UBE3B antibody (ab83834) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 123 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

Anti-UBE3B antibody (ab83834)参考文献

ab83834 has not yet been referenced specifically in any publications.

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