产品名称 Anti-UBE3B抗体 参阅全部 UBE3B 一抗
特异性 ab74653 detects endogenous levels of total UBE3B protein.
WB, ELISA more details
Synthetic peptide derived from an internal sequence of human UBE3B.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab74653 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 123 kDa.
相关性 UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
Anti-UBE3B antibody 图像
Western blot - UBE3B antibody (ab74653)
All lanes : Anti-UBE3B antibody (ab74653) at 1/500 dilution Lane 1 : Jurkat cell extracts Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 123 kDa Observed band size : 123 kDa Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.
Anti-UBE3B antibody (ab74653)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"