Anti-UBE2V1抗体(ab88679)

概述

  • 产品名称Anti-UBE2V1抗体
    参阅全部 UBE2V1 一抗
  • 描述
    小鼠多克隆抗体to UBE2V1
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human UBE2V1 (AAH00468).

  • 阳性对照
    • Human pancreas tissue lysate, UBE2V1 transfected 293T cell lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
  • 组织特异性Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Nucleus. Excluded from the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIR1 antibody
    • CROC-1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UB2V1_HUMAN antibody
    • UBE2V 1 antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin conjugating enzyme E2 variant 1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV-1 antibody
    • UEV1 antibody
    see all

Anti-UBE2V1 antibody 图像

  • Immunohistochemistical detection of Anti-UBE2V1 antibody (ab88679; 1/50 for 1h @ 21°C) on human kidney formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 0.25% Casein in PBS for 10 mins at 21°C. Secondary antibody HRP conjugated Dako REAL EnVision Goat anti Rabbit/Mouse (undiluted).

    See Abreview

  • Anti-UBE2V1 antibody (ab88679) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 32 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-UBE2V1 antibody (ab88679) at 1 µg/ml

    Lane 1 : UBE2V1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab88679 at 10µg/ml staining UBE2V1 in HeLa cells by Immunofluorescence.

Anti-UBE2V1 antibody (ab88679)参考文献

ab88679 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Casein in PBS as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Sample Human Tissue sections (Kidney)
Specification Kidney
Permeabilization Yes - 0.25% Triton X-100
Fixative Formaldehyde
Username

Dr. Francesca Conserva

Verified customer

提交于 May 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"