概述

  • 产品名称Anti-UBE2V1抗体
    参阅全部 UBE2V1 一抗
  • 描述
    兔多克隆抗体to UBE2V1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Synthetic peptide corresponding to Human UBE2V1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q13404

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas tissue lysate as well as the following whole cell lysates: PANC1; HeLa; U20S; HEK293; HeLa Nuclear.

性能

应用

Our Abpromise guarantee covers the use of ab101476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

靶标

  • 功能Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
  • 组织特异性Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Nucleus. Excluded from the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CIR1 antibody
    • CROC-1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UB2V1_HUMAN antibody
    • UBE2V 1 antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin conjugating enzyme E2 variant 1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV-1 antibody
    • UEV1 antibody
    see all

Anti-UBE2V1 antibody 图像

  • All lanes : Anti-UBE2V1 antibody (ab101476) at 1 µg/ml

    Lane 1 : Pancreas (Human) Tissue Lysate - adult normal tissue
    Lane 2 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 70 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab101476 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-UBE2V1 antibody (ab101476)参考文献

ab101476 has not yet been referenced specifically in any publications.

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