Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human UBE2U.
(Peptide available as
Liver (Human) Tissue Lysate - adult normal tissue;
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Use at an assay dependent concentration.
Catalyzes the covalent attachment of ubiquitin to other proteins.
Protein modification; protein ubiquitination.
Belongs to the ubiquitin-conjugating enzyme family.
Information by UniProt
Western blot - Anti-UBE2U antibody (ab76653)
Anti-UBE2U antibody (ab76653) at 1/250 dilution + Human liver tissue lysate - total protein (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Immunocytochemistry/ Immunofluorescence - Anti-UBE2U antibody (ab76653)
ICC/IF image of ab76653 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76653, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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