Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent dilution.
功能The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the ubiquitin-conjugating enzyme family.
翻译后修饰Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
ab117090, at 2.5 µg/ml staining UBE2N / Ubc13 in Human Thyroid cells by Immunohistochemistry. Tissue was Formalin-Fixed, Paraffin-Embedded (FFPE) after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with ab117090, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Anti-Ube2N / Ubc13 antibody (ab117090)参考文献
has not yet been referenced specifically in any publications.