RabMAb

Anti-UBE2M抗体[EPR5334] (ab124946)

概述

  • 产品名称Anti-UBE2M抗体[EPR5334]
    参阅全部 UBE2M 一抗
  • 描述
    兔单克隆抗体[EPR5334] to UBE2M
  • 经测试应用适用于: WB, ICCmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human UBE2M

  • 阳性对照
    • Human 293T and Ramos cell line lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
    • 通路Protein modification; protein neddylation.
    • 序列相似性Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
    • 结构域Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hUbc12 antibody
      • NEDD8 carrier protein antibody
      • NEDD8 conjugating enzyme Ubc12 antibody
      • NEDD8 protein ligase antibody
      • NEDD8-conjugating enzyme Ubc12 antibody
      • UBC-RS2 antibody
      • UBC12 antibody
      • UBC12_HUMAN antibody
      • ube2m antibody
      • ubiquitin carrier protein M antibody
      • Ubiquitin conjugating enzyme E2 M antibody
      • Ubiquitin-conjugating enzyme E2 M antibody
      • ubiquitin-protein ligase M antibody
      • yeast UBC12 homolog antibody
      see all

    Anti-UBE2M antibody [EPR5334] 图像

    • All lanes : Anti-UBE2M antibody [EPR5334] (ab124946) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 21 kDa

    Anti-UBE2M antibody [EPR5334] (ab124946)参考文献

    ab124946 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124946.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"