重组Anti-UBE2M/UBC12抗体[EPR5334] (ab124946)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5334] to UBE2M/UBC12
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UBE2M/UBC12抗体[EPR5334]
参阅全部 UBE2M/UBC12 一抗 -
描述
兔单克隆抗体[EPR5334] to UBE2M/UBC12 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human UBE2M/UBC12 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Human 293T and Ramos cell line lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5334 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124946于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
靶标
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功能
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. -
通路
Protein modification; protein neddylation. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. -
结构域
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8. - Information by UniProt
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数据库链接
- Entrez Gene: 9040 Human
- Entrez Gene: 22192 Mouse
- Entrez Gene: 361509 Rat
- Omim: 603173 Human
- SwissProt: P61081 Human
- SwissProt: P61082 Mouse
- Unigene: 406068 Human
- Unigene: 196580 Mouse
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别名
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124946 被引用在 1 文献中.
- Ba C et al. Ubiquitin conjugating enzyme E2 M promotes apoptosis in osteoarthritis chondrocytes via Wnt/ß-catenin signaling. Biochem Biophys Res Commun 529:970-976 (2020). PubMed: 32819607