概述

  • 产品名称Anti-UBE2M抗体
    参阅全部 UBE2M 一抗
  • 描述
    兔多克隆抗体to UBE2M
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    RGGYIGSTYFER

    , corresponding to amino acids 169-180 of Human UBE2M, conjugated to KLH.

  • 阳性对照
    • IHC-P: Human kidney tissue

性能

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应用

Our Abpromise guarantee covers the use of ab116248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.
ELISA 1/2000 - 1/10000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • 通路Protein modification; protein neddylation.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • 结构域Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hUbc12 antibody
    • NEDD8 carrier protein antibody
    • NEDD8 conjugating enzyme Ubc12 antibody
    • NEDD8 protein ligase antibody
    • NEDD8-conjugating enzyme Ubc12 antibody
    • UBC-RS2 antibody
    • UBC12 antibody
    • UBC12_HUMAN antibody
    • ube2m antibody
    • ubiquitin carrier protein M antibody
    • Ubiquitin conjugating enzyme E2 M antibody
    • Ubiquitin-conjugating enzyme E2 M antibody
    • ubiquitin-protein ligase M antibody
    • yeast UBC12 homolog antibody
    see all

Anti-UBE2M antibody 图像

  • ab116248, at 2.5 µg/ml, staining UBE2M in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-UBE2M antibody (ab116248)参考文献

ab116248 has not yet been referenced specifically in any publications.

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