Anti-UBE2I / UBC9抗体(ab21193)

概述

  • 产品名称
    Anti-UBE2I / UBC9抗体
    参阅全部 UBE2I / UBC9 一抗
  • 描述
    山羊多克隆抗体to UBE2I / UBC9
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Hamster, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    SGIALSRLAQERK

    , corresponding to N terminal amino acids 2-14 of Human Ube2I.

  • 阳性对照
    • Human Kidney, Human Lung and Human Testis lysates
  • 常规说明
    Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.

性能

应用

Our Abpromise guarantee covers the use of ab21193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. See Abreview.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.
ELISA 1/64000.

靶标

  • 功能
    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
  • 组织特异性
    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
  • 通路
    Protein modification; protein sumoylation.
  • 序列相似性
    Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位
    Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C358B7.1 antibody
    • p18 antibody
    • SUMO 1 protein ligase antibody
    • SUMO conjugating enzyme UBC9 antibody
    • SUMO-conjugating enzyme UBC9 antibody
    • SUMO-protein ligase antibody
    • SUMO1 protein ligase antibody
    • UBC9 antibody
    • UBC9_HUMAN antibody
    • UBCE9 antibody
    • Ube2i antibody
    • Ubiquitin carrier protein 9 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein I antibody
    • Ubiquitin conjugating enzyme 9 antibody
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody
    • Ubiquitin conjugating enzyme UbcE2A antibody
    • Ubiquitin like protein SUMO 1 conjugating enzyme antibody
    • Ubiquitin protein ligase E2I antibody
    • Ubiquitin-conjugating enzyme E2 I antibody
    • Ubiquitin-protein ligase I antibody
    see all

Anti-UBE2I / UBC9 antibody 图像

  • Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg

    Predicted band size : 18 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.

    See Abreview

Anti-UBE2I / UBC9 antibody (ab21193)参考文献

This product has been referenced in:
  • Xu J  et al. BRCA1 Mutation Leads to Deregulated Ubc9 Levels which Triggers Proliferation and Migration of Patient-Derived High Grade Serous Ovarian Cancer and Triple Negative Breast Cancer Cells. Int J Chronic Dis Ther 2:31-38 (2016). WB . Read more (PubMed: 28164176) »
  • Gross TJ  et al. A microRNA processing defect in smokers' macrophages is linked to SUMOylation of the endonuclease DICER. J Biol Chem 289:12823-34 (2014). WB ; Human . Read more (PubMed: 24668803) »

See all 8 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblast)
Loading amount
10 µg
Specification
Human fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (oocyte, embryo)
Specification
oocyte, embryo
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 25°C
Username

Dr. Motomasa Ihara

Verified customer

提交于 Nov 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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