The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 1-5 µg/ml. IF/ICC: Use at a concentration of 10 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the ubiquitin-conjugating enzyme family.