概述
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产品名称Ube2G1 overexpression 293T裂解物(whole cell)
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常规说明
ab94306 is a 293T cell transfected lysate in which Human Ube2G1 has been transiently over-expressed using a pCMV-Ube2G1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用适用于: WBmore details
性能
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形式Liquid
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存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
纯度Whole Cell Lysate
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研究领域
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背景Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Tissue specificity: Widely expressed, mainly in skeletal muscle. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.
应用
Our Abpromise guarantee covers the use of ab94306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
图片
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SDS-PAGE - Ube2G1 293T Transfected Lysate - (positive control) (ab94306)ab94306 at 15µg/lane on an SDS-PAGE gel. -
Western blot - Ube2G1 293T Transfected Lysate - (positive control) (ab94306)All lanes : Anti-Ube2G1 antibody (ab58256) at 1/500 dilution
Lane 1 :Ube2G1 overexpression 293T lysate (whole cell) (ab94306)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
文献
ab94306 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"