The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.
Protein modification; protein ubiquitination.
Belongs to the ubiquitin-conjugating enzyme family.
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.
ab71640 at 1/50 dilution, staining UBE2E3 in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.