重组Anti-UBE2D3抗体[EPR13001(B)] - N-terminal (ab176568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13001(B)] to UBE2D3 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UBE2D3抗体[EPR13001(B)] - N-terminal
参阅全部 UBE2D3 一抗 -
描述
兔单克隆抗体[EPR13001(B)] to UBE2D3 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human UBE2D3 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P61077 -
阳性对照
- MCF7, Jurkat and 293T cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13001(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176568于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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IP |
1/10 - 1/100.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
细胞定位
Cell membrane. Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7323 Human
- Entrez Gene: 66105 Mouse
- Entrez Gene: 81920 Rat
- Omim: 602963 Human
- SwissProt: P61077 Human
- SwissProt: P61079 Mouse
- SwissProt: P61078 Rat
- Unigene: 518773 Human
see all -
别名
- E2(17)KB3 antibody
- MGC43926 antibody
- MGC5416 antibody
see all
图片
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All lanes : Anti-UBE2D3 antibody [EPR13001(B)] - N-terminal (ab176568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa -
Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling UBE2D3 with ab176568 at 1/10 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab176568 被引用在 4 文献中.
- Du MG et al. The Absence of PTEN in Breast Cancer Is a Driver of MLN4924 Resistance. Front Cell Dev Biol 9:667435 (2021). PubMed: 33996822
- Pan Z et al. UBE2D3 Activates SHP-2 Ubiquitination to Promote Glycolysis and Proliferation of Glioma via Regulating STAT3 Signaling Pathway. Front Oncol 11:674286 (2021). PubMed: 34195079
- Shi Y et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). WB ; Human . PubMed: 28469175
- Yang H et al. Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells. J Cancer 7:1152-62 (2016). WB . PubMed: 27326259