A region within synthetic peptide: ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDREKYNRIA REWTQKYAM, corresponding to C terminal amino acids 99-147 of Human UBE2D2
Our Abpromise guarantee covers the use of ab66602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:1562500. |
|
IHC-P | Use a concentration of 4 - 8 µg/ml. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBE2D2 with ab66602 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
ab66602 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"