Anti-UBE2D2抗体(ab66602)

概述

  • 产品名称Anti-UBE2D2抗体
    参阅全部 UBE2D2 一抗
  • 描述
    兔多克隆抗体to UBE2D2
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDREKYNRIA REWTQKYAM, corresponding to C terminal amino acids 99-147 of Human UBE2D2

  • 阳性对照
    • Jurkat cells. Lung.

性能

应用

Our Abpromise guarantee covers the use of ab66602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E2(17)KB2 antibody
    • OTTHUMP00000223475 antibody
    • OTTHUMP00000223476 antibody
    • OTTHUMP00000223477 antibody
    • OTTHUMP00000223478 antibody
    • OTTHUMP00000224375 antibody
    • PUBC 1 antibody
    • PUBC1 antibody
    • UB2D2_HUMAN antibody
    • UBC 4 antibody
    • UBC 4/5 antibody
    • UBC4 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBCH 5B antibody
    • UBCH5B antibody
    • UBE2D2 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D2 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 2 antibody
    • Ubiquitin conjugating enzyme E2 D2 antibody
    • Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 2 antibody
    • Ubiquitin protein ligase D2 antibody
    • Ubiquitin-conjugating enzyme E2 D2 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 2 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody
    • Ubiquitin-protein ligase D2 antibody
    see all

Anti-UBE2D2 antibody 图像

  • Anti-UBE2D2 antibody (ab66602) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBE2D2 with ab66602 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.



Anti-UBE2D2 antibody (ab66602)参考文献

ab66602 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The C. elegans ortholog is called LEThal family member (let-70). Here is a link to the NCBI record for the protein: http://www.ncbi.nlm.nih.gov/protein/P35129.1 . I hope this information is helpful to ...

Read More

Thank you for contacting us. My BLAST search of the amino acid range listed in the immunogen field on the datasheet just turned up a gene from C. vulgaris, Cre-Let-70. I am going to ask the laboratory that produces the antibody to help with this one an...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"