概述
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产品名称Anti-UBE2D2抗体
参阅全部 UBE2D2 一抗 -
描述兔多克隆抗体to UBE2D2
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宿主Rabbit
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经测试应用适用于: WB, ELISA, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Dog, Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
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免疫原
A region within synthetic peptide: ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDREKYNRIA REWTQKYAM, corresponding to C terminal amino acids 99-147 of Human UBE2D2
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阳性对照
- Jurkat cells. Lung.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab66602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:1562500. |
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| IHC-P | Use a concentration of 4 - 8 µg/ml. |
靶标
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功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
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通路Protein modification; protein ubiquitination.
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序列相似性Belongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
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数据库链接
- Entrez Gene: 7322 Human
- Entrez Gene: 56550 Mouse
- Entrez Gene: 641452 Rat
- Omim: 602962 Human
- SwissProt: P62837 Human
- SwissProt: P62838 Mouse
- SwissProt: P62839 Rat
- Unigene: 108332 Human
see all -
别名
- E2(17)KB2 antibody
- OTTHUMP00000223475 antibody
- OTTHUMP00000223476 antibody
see all
图片
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Western blot - Anti-UBE2D2 antibody (ab66602)Anti-UBE2D2 antibody (ab66602) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 15% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2D2 antibody (ab66602)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBE2D2 with ab66602 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
文献
ab66602 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"