antigen sequence: SLVIESLRNS KYIKKFPLLE NTFKADSSSA WSAVKVLYQP CIKSRNEKLV SLWESDISVH PLTLPSATCL ELLL, corresponding to amino acids of Human UBE2CBP.
- Human heart muscle tissue; RT 4, U 251 MG, Human liver and tonsil lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.
Protein modification; protein ubiquitination.
The C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
Information by UniProt
- C6orf157 antibody
- chromosome 6 open reading frame 157 antibody
- DKFZp434A1520 antibody
Immunocytochemistry/ Immunofluorescence - Anti-UBE2CBP antibody (ab121927)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121927 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-UBE2CBP antibody (ab121927)
All lanes : Anti-UBE2CBP antibody (ab121927) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2CBP antibody (ab121927)
ab121927, at 1/20, staining UBE2CBP in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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