概述

  • 产品名称Anti-UBE2CBP抗体
  • 描述
    兔多克隆抗体to UBE2CBP
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: SLVIESLRNS KYIKKFPLLE NTFKADSSSA WSAVKVLYQP CIKSRNEKLV SLWESDISVH PLTLPSATCL ELLL, corresponding to amino acids of Human UBE2CBP.

  • 阳性对照
    • Human heart muscle tissue; RT 4, U 251 MG, Human liver and tonsil lysate.

性能

应用

Our Abpromise guarantee covers the use of ab121927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.
  • 通路Protein modification; protein ubiquitination.
  • 结构域The C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
  • 翻译后修饰Ubiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C6orf157 antibody
    • chromosome 6 open reading frame 157 antibody
    • DKFZp434A1520 antibody
    • EC 6.3.2 antibody
    • EC 6.3.2.- antibody
    • H10BH antibody
    • MGC126561 antibody
    • MGC126587 antibody
    • OTTHUMP00000016789 antibody
    • OTTHUMP00000220801 antibody
    • UB2CB_HUMAN antibody
    • UBCH10 binding protein with a hect-like domain antibody
    • UbcH10-binding protein with a HECT-like domain antibody
    • UBE2CBP antibody
    • Ubiquitin-conjugating enzyme E2C-binding protein antibody
    • YJR141W antibody
    see all

Anti-UBE2CBP antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121927 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-UBE2CBP antibody (ab121927) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab121927, at 1/20, staining UBE2CBP in paraffin embedded Human heart muscle tissue by Immunohistochemistry.

Anti-UBE2CBP antibody (ab121927)参考文献

ab121927 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121927.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"