概述

  • 产品名称Anti-UBE2C抗体
    参阅全部 UBE2C 一抗
  • 描述
    兔多克隆抗体to UBE2C
  • 特异性This antibody recognises a band of the correct size (20 kDa) in Hela, A431, Jurkat, Jurkat nuclear and 293 lysates.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from within residues 150 to the C-terminus of Human UBE2C.

    .

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; HEK293. It also gave a positive signal in Jurkat nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab12290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500. Predicted molecular weight: 20 kDa.
IHC-P 1/100.

靶标

  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 翻译后修饰Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cyclin selective ubiquitin carrier protein antibody
    • dJ447F3.2 antibody
    • Mitotic specific ubiquitin conjugating enzyme antibody
    • UB E2C antibody
    • UBCH 10 antibody
    • UbcH10 antibody
    • UBE 2C antibody
    • Ube2c antibody
    • UBE2C_HUMAN antibody
    • Ubiquitin carrier protein C antibody
    • Ubiquitin carrier protein E2 C antibody
    • Ubiquitin carrier protein E2C antibody
    • Ubiquitin conjugating enzyme E2 C antibody
    • Ubiquitin conjugating enzyme E2C antibody
    • Ubiquitin protein ligase C antibody
    • Ubiquitin-conjugating enzyme E2 C antibody
    • Ubiquitin-protein ligase C antibody
    see all

Anti-UBE2C antibody 图像

  • Image courtesy of Human Protein Atlas
    ab12290 stainning UBEC2 in the nucleus of surface epithelial cells of human esophagus. Paraffin-embbeded tissue was incubated with ab12290 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
    ab12290 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
  • All lanes : Anti-UBE2C antibody (ab12290) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Jurkat nuclear extract lysate (ab14844)
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 Human embryonic kidney cell line Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 55 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab12290 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab12290, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 and MCF7 cells.

Anti-UBE2C antibody (ab12290)参考文献

This product has been referenced in:
  • García-Escudero R  et al. Gene expression profiling of mouse p53-deficient epidermal carcinoma defines molecular determinants of human cancer malignancy. Mol Cancer 9:193 (2010). IHC-P ; Human . Read more (PubMed: 20630075) »
  • Lin J  et al. Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on esophageal adenocarcinoma. Neoplasia 8:1062-71 (2006). Read more (PubMed: 17217624) »

See all 2 Publications for this product

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