Ube2B overexpression 293T裂解液(全细胞) (ab94308)

概述

  • 产品名称Ube2B overexpression 293T裂解液(全细胞)
  • 描述
    Ube2B overexpression裂解液
  • 常规说明ab94308 is a 293T cell transfected lysate in which Human Ube2B has been transiently over-expressed using a pCMV-Ube2B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.

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应用

Our Abpromise guarantee covers the use of ab94308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Ube2B overexpression 293T lysate (whole cell) 图像

  • ab94308 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Ube2B antibody (ab67118) at 1/500 dilution

    Lane 1 : Ube2B 293T Transfected Lysate - Positive Control (ab94308)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

Ube2B overexpression 293T lysate (whole cell) (ab94308)参考文献

ab94308 has not yet been referenced specifically in any publications.

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