概述

  • 产品名称Anti-Ube2B抗体
    参阅全部 Ube2B 一抗
  • 描述
    兔多克隆抗体to Ube2B
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1 and 152 of Human Ube2B (NP_003328)

  • 阳性对照
    • HeLa and NIH-3T3 whole cell lysate. HepG2 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab96848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.

靶标

  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E2 17kDa antibody
    • E2 protein antibody
    • hHR6B antibody
    • HR6B antibody
    • RAD6 homolog B antibody
    • RAD6B antibody
    • UBC2 antibody
    • UBE2B antibody
    • UBE2B_HUMAN antibody
    • Ubiquitin carrier protein B antibody
    • Ubiquitin conjugating enzyme E2 B antibody
    • Ubiquitin conjugating enzyme E2B (RAD6 homolog) antibody
    • Ubiquitin conjugating enzyme E2B antibody
    • Ubiquitin protein ligase B antibody
    • Ubiquitin-conjugating enzyme E2 17 kDa antibody
    • Ubiquitin-conjugating enzyme E2 B antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa antibody
    • Ubiquitin-protein ligase B antibody
    see all

Anti-Ube2B antibody 图像

  • Anti-Ube2B antibody (ab96848) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 17 kDa
  • Anti-Ube2B antibody (ab96848) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 17 kDa

Anti-Ube2B antibody (ab96848)参考文献

ab96848 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"