重组Anti-Ube1L抗体[EPR4270] (ab108929)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4270] to Ube1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ube1L抗体[EPR4270]
参阅全部 Ube1L 一抗 -
描述
兔单克隆抗体[EPR4270] to Ube1L -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, HL60, HeLa, Jurkat, and human fetal liver cell lysate. GST tagged Recombinant human UBA7(Ube1L) protein. ICC/IF: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4270 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108929于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
组织特异性
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-activating E1 family. - Information by UniProt
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数据库链接
- Entrez Gene: 7318 Human
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
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别名
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
see all
图片
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All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa -
All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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