概述

  • 产品名称
  • 描述
    兔多克隆抗体to Ube1L
  • 特异性
    The specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not. This antibody reacts with the C-terminus of Ube1L.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide:

    PGSQDWTALRELLKLL

    , corresponding to amino acids 741-756 of Human Ube1L.

性能

应用

Our Abpromise guarantee covers the use of ab12200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 112 kDa.

靶标

  • 功能
    Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • 组织特异性
    Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D8 antibody
    • MGC12713 antibody
    • UBA1, ubiquitin activating enzyme E1 homolog B antibody
    • UBA1B antibody
    • UBA7 antibody
    • UBA7, ubiquitin activating enzyme E1 antibody
    • UBA7_HUMAN antibody
    • Ube 1L antibody
    • UBE 2 antibody
    • UBE1L antibody
    • UBE2 antibody
    • Ubiquitin activating enzyme 2 antibody
    • Ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin Activating Enzyme E1 Like antibody
    • Ubiquitin activating enzyme E1 related protein antibody
    • Ubiquitin-activating enzyme 7 antibody
    • Ubiquitin-activating enzyme E1 homolog antibody
    • ubiquitin-activating enzyme-2 antibody
    • ubiquitin-like modifier activating enzyme 7 antibody
    • Ubiquitin-like modifier-activating enzyme 7 antibody
    see all

图片

  • Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).

文献

This product has been referenced in:
  • Kitareewan S  et al. UBE1L is a retinoid target that triggers PML/RARalpha degradation and apoptosis in acute promyelocytic leukemia. Proc Natl Acad Sci U S A 99:3806-11 (2002). Read more (PubMed: 11891284) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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