概述

  • 产品名称Anti-Ube1L抗体
    参阅全部 Ube1L 一抗
  • 描述
    兔多克隆抗体to Ube1L
  • 特异性The specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not. This antibody reacts with the N-terminus of Ube1L.
  • 经测试应用适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide:

    DCDPRSIHVREDGSLEIGD

    , corresponding to amino acids 222-240 of Human Ube1L.

性能

应用

Our Abpromise guarantee covers the use of ab12199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/500. Detects a band of approximately 112 kDa.

靶标

  • 功能Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • 组织特异性Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D8 antibody
    • MGC12713 antibody
    • UBA1, ubiquitin activating enzyme E1 homolog B antibody
    • UBA1B antibody
    • UBA7 antibody
    • UBA7, ubiquitin activating enzyme E1 antibody
    • UBA7_HUMAN antibody
    • Ube 1L antibody
    • UBE 2 antibody
    • UBE1L antibody
    • UBE2 antibody
    • Ubiquitin activating enzyme 2 antibody
    • Ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin Activating Enzyme E1 Like antibody
    • Ubiquitin activating enzyme E1 related protein antibody
    • Ubiquitin-activating enzyme 7 antibody
    • Ubiquitin-activating enzyme E1 homolog antibody
    • ubiquitin-activating enzyme-2 antibody
    • ubiquitin-like modifier activating enzyme 7 antibody
    • Ubiquitin-like modifier-activating enzyme 7 antibody
    see all

Anti-Ube1L antibody 图像

  • Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).
  • Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.

    This picture was kindly supplied as part of the review submitted by Sonia del Rincon.

Anti-Ube1L antibody (ab12199)参考文献

This product has been referenced in:
  • Chan EY  et al. Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production. J Virol 81:7571-83 (2007). WB ; Human . Read more (PubMed: 17494070) »
  • Pitha-Rowe I  et al. Involvement of UBE1L in ISG15 Conjugation during Retinoid-induced Differentiation of Acute Promyelocytic Leukemia. J Biol Chem 279:18178-87 (2004). Read more (PubMed: 14976209) »

See all 3 Publications for this product

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