概述

  • 产品名称
  • 描述
    兔多克隆抗体to Ube1L
  • 特异性
    The specificity of this antibody has been confirmed by immunoblot analyses of Ube1L-transfected CHO cells. CHO cells that did not basally express Ube1L mRNA were transfected with a full-length Ube1L cDNA or an insertless vector. CHO cells transfected with Ube1L expressed Ube1L, whereas cells transfected with an insertless vector did not. This antibody reacts with the N-terminus of Ube1L.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide:

    DCDPRSIHVREDGSLEIGD

    , corresponding to amino acids 222-240 of Human Ube1L.

性能

应用

Our Abpromise guarantee covers the use of ab12199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/500. Detects a band of approximately 112 kDa.

靶标

  • 功能
    Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • 组织特异性
    Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D8 antibody
    • MGC12713 antibody
    • UBA1, ubiquitin activating enzyme E1 homolog B antibody
    • UBA1B antibody
    • UBA7 antibody
    • UBA7, ubiquitin activating enzyme E1 antibody
    • UBA7_HUMAN antibody
    • Ube 1L antibody
    • UBE 2 antibody
    • UBE1L antibody
    • UBE2 antibody
    • Ubiquitin activating enzyme 2 antibody
    • Ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin Activating Enzyme E1 Like antibody
    • Ubiquitin activating enzyme E1 related protein antibody
    • Ubiquitin-activating enzyme 7 antibody
    • Ubiquitin-activating enzyme E1 homolog antibody
    • ubiquitin-activating enzyme-2 antibody
    • ubiquitin-like modifier activating enzyme 7 antibody
    • Ubiquitin-like modifier-activating enzyme 7 antibody
    see all

图片

  • Immunohistochemistry using ab12200 (C-terminus) and ab12199 (N-terminus) with peptide blocking (right panel) and without peptide blocking (left panel).
  • Immunofluorescence was used to detect UBE1L protein expression in MDA-MB-231 cells (red staining) and DAPI staining revealed the nuclei (blue staining). The right panel shows immunofluorescence with no primary antibody.

    This picture was kindly supplied as part of the review submitted by Sonia del Rincon.

文献

This product has been referenced in:
  • Ruosaari S  et al. Pathways affected by asbestos exposure in normal and tumour tissue of lung cancer patients. BMC Med Genomics 1:55 (2008). Read more (PubMed: 19014429) »
  • Chan EY  et al. Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production. J Virol 81:7571-83 (2007). WB ; Human . Read more (PubMed: 17494070) »

See all 4 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab12199.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册